Figure 3. Hexafluoro attenuates TGF-β-induced fibrotic gene expression.

Lung fibroblasts were preincubated with hexafluoro (10 μM) for 30 min, followed by TGF-β2 (10 ng/ml) for 24 h. A. Relative mRNA levels were examined by real-time qPCR. Results normalized with GAPDH are means ±SD of triplicate determinations from a representative experiment of three. *, p < 0.05. B. Whole cell lysates were examined by Western analysis. Representative images. Cgn I, Type I collagen. C. Left, Confocal immunofluorescence using antibodies to αSMA (green) and Type I collagen (red). Bar = 10μm. Right, Quantification of immunofluorescence intensity. Results are the means ±SD of triplicate experiments.