Figure 7. Combination effect of N6L and docetaxel on tumor growth in vitro and in vivo.

A-C. Cells were treated for 72 hours with N6L and/or docetaxel. Cell viability was then measured using MTT staining. A, Inhibition of LNCaP and LNCaP-NE cell viability with docetaxel treatment. B and C, Effect of the combination N6L/docetaxel on LNCaP (B) and LNCaP-NE (C) cell viability. D and E. Effect of 50 μmol/L N6L, 100 nmol/L docetaxel and the combination 50 μmol/L N6L/100 nmol/L docetaxel on LNCaP (D) and LNCaP-NE (E) colony viability in soft agar. Cells were treated for 72 hours and colony viability was measured by MTT staining (alive cells appeared in dark/violet). A colony was considered as dead when 50 % of the cells forming the colony were dead (white cells). A picture representing results for each condition is represented above the corresponding graph bar. Scale bar, 100 μm. F. Effect of N6L, docetaxel and the combination on tumor growth in vivo in DU145 ectopic xenografts in nude mice. Mice bearing tumors of approximatively 100 mm³ were treated by intra-peritoneal injections three times per week with PBS (control group) or 10 mg/kg N6L and/or two times per week with 5 mg/kg docetaxel (n = 8 per group).