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. 2016 Aug 31;7(43):69536–69548. doi: 10.18632/oncotarget.11766

Figure 1. Endogenous MXD1 localizes in the nucleolus.

Figure 1

(A) MXD1 protein expression in growing cells from different cell lines used in this work. MXD1 levels were analyzed by immunoblot in the indicated cell lines (left panel). The right panel shows MXD1 expression in HeLa cells deprived of serum for 48 h. The expression of β-tubulin or α-actin levels were determined as a control for protein loading. (B) MXD1 localization in the nucleolus. Cells from the indicated human cell lines and rat sensory neurons were double immunolabelled for MXD1 (red) and UBF (green). MXD1 showed a nuclear distribution, including the nucleolus where colocalized with UBF. Scale bars: 5 μm.