Figure 3. Ectopic MXD1 localizes in nucleoli.

(A) Nucleolar localization of transfected MXD1. HeLa cells transfected with the GFP, GFP-MNT and GFP-MXD1 constructs (green). Whereas GFP-MXD1 protein localized in the nucleolus, GFP-MNT showed a nuclear staining but was absent from the nucleolus. (B) HeLa cells were transfected with the GFP-MXD1 construct and 24 h after transfection immunofluorescence was performed for endogenous UBF as described in Figure 1 with a secondary antibody conjugated with Texas Red. Nuclei were stained with DAPI (blue). The images were acquired by confocal (A) or epifluorescence (B) microscopy. Scale bars: 5 μm.