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. 2016 Sep 6;7(43):69592–69605. doi: 10.18632/oncotarget.11870

Figure 3. EPRS is necessary for proliferation ER+, but not ER− breast cancer cells.

Figure 3

(A) EPRS knockdown inhibits growth of MCF7 TamR cells in 3D Matrigel™ culture. (B) Flow cytometric analysis of PI-stained DNA. Numbers are mean percentages from three independent experiments. Color ring represents -log10 (student's t-test p values). C&D) Representative immunoblot of G1/S/G2 proteins in MCF7 TamR (C) and parental (D) cells treated with EPRS or control siRNA in the absence and presence of 20 μM Z-VAD-FMK. (E) EPRS knockdown does not inhibit growth of ER- MDA-MB-453 cells in 3D Matrigel™ culture. Representative immunoblot of EPRS knockdown in MDA-MB-453 cells. (F) Flow cytometric cell cycle analysis of PI-stained MDA-MB-453 cells. Numbers are mean percentages from three independent experiments. siLuc: luciferase-targeting siRNA (control). siEPRS: pool of four unique siEPRS-targeting siRNAs.