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. 2016 Sep 8;7(43):69625–69637. doi: 10.18632/oncotarget.11904

Figure 6. GSK2830371 enhances p53 activation and apoptosis induction by Nutlin-3a, bortezomib, and doxorubicin.

Figure 6

Z-138 and JVM-2 cells were treated for 24 hours with GSK2830371 (4 μM for Z-138 and 10 μM for JVM-2) or (A) Nutlin-3a (2 μM for Z-138 and 6 μM JVM-2), (B) bortezomib (12 nM for Z-138 and 14 nM for JVM-2), or (C) doxorubicin (16 nM Z-138 and 100 nM JVM-2) either as individual agents or in combination, after which the levels of total and phosphorylated p53 and PARP were determined. (D) MAVER-1 cells were treated with 10 μM GSK2830371 and 14 nM bortezomib either as individual agents or in combination. The expression levels of total and phosphorylated p53 were determined after 24 hours of treatment; the degree of PARP cleavage was determined after 48 hours. The intensities of immunoblot signals were quantified, the intensities relative to β-actin were calculated, and the levels in untreated cells were set at 1.0. The results are representative of three independent experiments.