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. 2016 Sep 10;7(43):69649–69665. doi: 10.18632/oncotarget.11935

Figure 2. Regulation of CDCP1 expression by WHF in TNBC cell lines.

Figure 2

(A) The TNBC cell lines MDA-MB-231, BT-549, SUM149, SUM159, MDA-MB-157, HCC1937 and MDA-MB-468, considered basal-like (B) or mesenchymal-like (M) per Lehmann classification (4), were starved (0% FBS) for 24 h, stimulated for 48 h with a WHF pool, and processed for western blot analysis of CDCP1 (the full-length 135-kD and 70-kD forms) using polyclonal anti-CDCP1. Monoclonal anti-actin was used as a loading control. The results are representative of 3 independent experiments. (B) The graph shows the fold-increase ± standard error of the mean (SEM) in full-length CDCP1 on WHF stimulation for each cell line in 3 western blot experiments, evaluated by densitometry and normalized to actin levels.