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. 2016 Sep 10;7(43):69649–69665. doi: 10.18632/oncotarget.11935

Figure 3. Effect of silencing CDCP1 on signaling mediators.

Figure 3

(A) MDA-MB-231 and BT-549 cells were transfected with a pool of 4 oligonucleotides (100 nM) that bind and degrade CDCP1 mRNA (see Materials and Methods for sequences) or with the appropriate negative control siRNAs. Cells were harvested at 48 h post-transfection, and CDCP1 expression was verified by western blot. Monoclonal anti-actin was used as a loading control. The knockdown efficiency was ≥ 60% for both CDCP1 forms. (B) CDCP1 siRNA-treated and control-treated MDA-MB-231 and BT-549 cells were analyzed for activation of CDCP1, Src, and PKCδ. Monoclonal anti-actin and anti-vinculin were used as loading controls.