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CDCP1 siRNA-treated and control-treated MDA-MB-231 and BT-549 cells were plated into Boyden chambers for the migration assay (A, B) and in Transwells that were coated with Matrigel for the invasion assay (C, D). The area occupied by migrated cells in the Transwell assay (mean ± SEM in 3 independent experiments) was measured by digital image analysis (Image Pro-Plus 7.0 application, Media Cybernetics). *p < 0.05.
