Figure 5. Effect of Rhiz on AR-V7, PSA, and IGF-1 protein and mRNA expression.
(A) Effect of Rhiz on AR-V7 expression in 22Rv1 and VCaP cells. Protein expression was analyzed by Western blotting (cropped blots), mRNA levels were analyzed by qPCR. (B) Effect of Rhiz on PSA expression in 22Rv1 and LNCaP cells. The concentration of PSA in the culture supernatant was analyzed using ELISA and normalized to the number of viable cells, mRNA levels were analyzed by qPCR. (C) Effect of Rhiz on IGF-1 expression in 22Rv1, VCaP, and LNCaP cells analyzed by Western blotting (cropped blots), mRNA levels were analyzed by qPCR.