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. 2016 Aug 24;7(43):69961–69975. doi: 10.18632/oncotarget.11572

Figure 2. Increased Btk, stemness marker expression and temozolomide resistance in GBM spheres.

Figure 2

A. Identification of CD133+ population of GBM cells using flow cytometric analysis. CD133+ cell population was identified in both U87MG and DTBRG-05MG cell lines (approximately 4.4% and 9.2% respectively). B. CD133+ GBM cells when cultured under serum-deprived conditions showed an increased propensity for GBM sphere formation as compared to their CD133- parental counterparts. C. Comparative Western blots analysis between GBM parental cells and GBM spheres. In both cell lines, Btk and oncogenic Akt/mTOR signaling expression were both found higher in the GBM spheres than their parental counterparts. D. GBM GBM spheres exhibited enhanced temozolomide resistance. The IC50 value was found to be approximately 300 and 500 μM for U87MG and DBTRG-05 cells respectively. U87MG and DBTRG-05 GBM spheres remained approximately 85.7% and 67.8% viable at the IC50 concentrations of their respective parental counterparts.