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. 2016 Aug 24;7(43):69961–69975. doi: 10.18632/oncotarget.11572

Figure 3. Btk-silencing resulted in decreased malignant GBM phenotypes and stemness.

Figure 3

A. Btk knocked down U87MG and DBTRG-05MG cells exhibited a significantly decreased colony-forming ability. B. Btk-silenced U87MG and DBTRG-05MG cells demonstrated a decreased migratory ability as compared to their counterparts. C. Down-regulation of Btk resulted in a significantly reduced sphere-forming ability in both cell lines. D. Flow cytometric analysis showed that Btk-silencing was associated with reduced the percentage of CD133+ U87MG and DBTRG-05MG (approximately 4.4% and 0.9% reduction respectively). E. Comparative Western blots between parental and Btk-knocked down cells. Btk-downregulation was associated with reduced expression level of stemness genes including c-Myc, Nestin, as well as the oncogenic Akt/mTOR signaling and the EMT marker, Vimentin.