Figure 7. FGFR4 silencing-induced damage persistence in HT29 cells.

Immunofluorescence of γ-H2AX foci post IR was performed as described in Materials and Methods, and Figure 4. After FGFR4 knockdown (A) or PD173074 treatment (B), cells were treated with a single 6 Gy dose of γ-rays and stained for γ-H2AX 24 h later. Upper panels show representative photographs, lower panels show the quantification of γ-H2AX-foci.