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. 2016 Sep 17;7(43):69976–69990. doi: 10.18632/oncotarget.12099

Figure 8. FGFR4 overexpression induced radioresistance.

Figure 8

FGFR4 was overexpressed in MMR-proficient SW480 cells and in MMR-deficient DLD1 cells and overexpression was verified by western blotting. After γ-irradiation the surviving cell fraction was increased in the FGFR4-SW480 cells (A), but not in the FGFR4-DLD1 cells (B). Western blot showing stabilization of RAD51 protein levels of irradiated FGFR4 overexpressing SW480 (C) and DLD1 (D) cells, as compared to pcDNA-transfected cells. RAD51 protein lysates were collected at 24, 48 and 72 h after single 6 Gy dose. Fluorescence staining of γ-H2AX in formalin fixed SW480 (E) and DLD1 (F) cells 24 h after irradiation with a single 6 Gy dose. Upper panels show representative pictures of γ-H2AX foci (green fluorescence), lower panels show the quantification of γ-H2AX foci relative to control. Quantification of the HR capacity in SW480 (G) and DLD1 (H) cells, represented by the ratio of GFP+ cells to DsRed+ cells, as described in the Matrials and Methods. The bars represent the mean of 3 independent cultures ± SEM.