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. 2016 Sep 20;7(43):70080–70091. doi: 10.18632/oncotarget.12148

Figure 2. Increased expression of FRMD6 inhibits the GBM cell proliferation and invasion through Matrigel.

Figure 2

(A) Establishment of U87MG and U251 GBM cells expressing v5 epitope tagged FRMD6 (U87MG/U251FRMD6) or transduced with empty retroviral expression vector alone (U87MG/U251control). FRMD6v5 was detected by anti-v5 mAb (Invitrogen). (B) GBM cell proliferation assays were performed every day using a set of 96-well plates and the Premix WST1 kit (TaKaRa) following the manufacturer's instruction. **p < 0.01. (C) Invasion capacity of the transduced GBM cells was assessed by using the Matrigel (Corning) coated transwell plates. Representative images of the GBM cells migrated through the Matrigel-coated transwell inserts are shown and the round holes represent 8-μm pores on transwell membranes. (D) GBM cells migrated through the Matrigel-coated transwell inserts in 30 random selected 100× microscopic filed were counted and their quantitative mean values with SD are shown. **p < 0.01.