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. 2016 Sep 21;7(43):70143–70151. doi: 10.18632/oncotarget.12160

Figure 2. ISG15 suppresses cancer cell proliferation.

Figure 2

(A) K562, OCI-AML2 and OPM2 cells were infected with lentiviral ISG15 for 96 hrs, followed by immunoblotting assay to evaluate ISG15 expression. (B) The above cells infected with lentiviral ISG15 or mock lentivirus for 96 hrs were subjected to cell viability analysis by MTT. (C) K562 cells were transfected with siISG15 and incubated for 48 hrs, followed by immunoblotting assay to evaluate ISG15 knockdown. (D, E) K562 cells transfected with siISG15 were subjected to CLQ (D) or MFQ (E) treatment for 72 hrs followed by MTT assay for cell proliferation.