Table 1. Levels of mRNA expression of clock genes in the PER1-shRNA-I, Control-shRNA and SCC15 groups in vitro (mean±SD).
| Gene | PER1-shRNA-I | Control-shRNA | SCC15 | F | P | P1 | P2 | P3 |
|---|---|---|---|---|---|---|---|---|
| PER1 | 0.25±0.09 | 1.00 | 1.23±0.29 | 26.09 | 0.001 | 0.002 | 0.000 | 0.156 |
| PER2 | 0.52±0.09 | 1.00 | 0.99±0.05 | 64.24 | 0.000 | 0.000 | 0.000 | 0.871 |
| PER3 | 1.28±0.11 | 1.00 | 0.92±0.09 | 14.64 | 0.005 | 0.007 | 0.002 | 0.259 |
| DEC1 | 0.65±0.08 | 1.00 | 1.05±0.02 | 56.02 | 0.000 | 0.000 | 0.000 | 0.293 |
| DEC2 | 0.51±0.06 | 1.00 | 1.03±0.11 | 50.21 | 0.000 | 0.000 | 0.000 | 0.665 |
| CRY1 | 0.82±0.09 | 1.00 | 1.05±0.05 | 12.34 | 0.007 | 0.011 | 0.003 | 0.317 |
| CRY2 | 0.70±0.05 | 1.00 | 1.03±0.05 | 53.08 | 0.000 | 0.000 | 0.000 | 0.476 |
| TIM | 1.36±0.07 | 1.00 | 0.97±0.03 | 87.12 | 0.000 | 0.000 | 0.000 | 0.401 |
| RORα | 2.02±0.66 | 1.00 | 1.16±0.14 | 6.05 | 0.036 | 0.018 | 0.034 | 0.639 |
| NPAS2 | 0.67±0.04 | 1.00 | 1.03±0.08 | 44.36 | 0.000 | 0.000 | 0.000 | 0.469 |
| REV-ERBα | 1.59±0.14 | 1.00 | 1.04±0.18 | 19.55 | 0.002 | 0.001 | 0.002 | 0.695 |
| CLOCK | 0.99±0.08 | 1.00 | 1.00±0.05 | 0.04 | 0.964 | 0.801 | 0.856 | 0.943 |
| BMAL1 | 0.98±0.07 | 1.00 | 0.95±0.09 | 0.37 | 0.705 | 0.693 | 0.670 | 0.422 |
| CKIε | 0.97±0.10 | 1.00 | 1.06±0.10 | 1.00 | 0.421 | 0.630 | 0.212 | 0.408 |
Notes: P values reflect differences in each gene expression among the three groups analyzed using one-way ANOVA.
P1, P2, and P3 respectively reflect the intergroup differences between the PER1-shRNA-I and Control-shRNA groups, the PER1-shRNA-I and SCC15 groups, and the Control-shRNA and SCC15 groups analyzed using the LSD test after one-way ANOVA. Values of P<0.05 were considered statistically significant.