Skip to main content
. 2016 Sep 2;7(43):70290–70302. doi: 10.18632/oncotarget.11844

Table 4. Primer sequences for clock genes used for quantitative real-time PCR.

Gene Forward primer sequence Reverse primer sequence
PER1 5′-CTGCTACAGGCACGTTCAAG-3′ 5′-CTCAGGGACCAAGGCTAGTG-3′
PER2 5′-TTGGACAGCGTCATCAGGTA-3′ 5′-TCCGCTTATCACTGGACCTT-3′
PER3 5′-GCAGGTCTATGCCAGTGTGA-3′ 5′-ACCACCACCATTCGGTTCT-3′
CLOCK 5′-CAGCCAGTGATGTCTCAAGC-3′ 5′-ATGCGTGTCCGTTGTTCC-3′
BMAL1 5′-TGCCACCAATCCATACACAG-3′ 5′-TTCCCTCGGTCACATCCTAC-3′
DEC1 5′-CAGCTTTCGGATGATGAAGG-3′ 5′-GCTGAAGGTGGGATCAGGTA-3′
DEC2 5′-GGGACCAACTGCTTCACACT-3′ 5′-TAATCTGTGGGACGGTAGGC-3′
CRY1 5′-TGTGATTCGTGGACAACCAG-3′ 5′-TAGCTGCGTCTCGTTCCTTT-3′
CRY2 5′-AGGAGAACCACGACGAGA-3′ 5′-TCCGCTTCACCTTTTTATAC-3′
CKIε 5′-TGAGTATGAGGCTGCACAGG-3′ 5′-CTTCCCGAGATGGTCAAATG-3′
TIM 5′-GATAGAGGCCCATTCCTGCAT-3′ 5′-GAAGGGCTGGGGAACTTAGAC-3′
NPAS2 5′-AACCTCGGCAGCACTTTAAC-3′ 5′-GGTTCTGACATGGCTGTGTG-3′
RORα 5′-CTATCCCTCCAAGGCACAAG-3′ 5′-AACACAAGACTGACGAGCACA-3′
REV-ERBα 5′-ACAGAATCGAACTCTGCACTTCT-3′ 5′-GGGGAGGGAGGCAGGTATT-3′
β-actin 5′-AGCGAGCATCCCCCAAAGTT-3′ 5′-GGGCACGAAGGCTCATCATT-3′