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. 2016 Sep 6;7(43):70303–70322. doi: 10.18632/oncotarget.11854

Figure 2. NTS/NTR1 co-expression was correlated with tumor invasion potentials of HCC cell lines.

Figure 2

a. The expression level of NTS was detected using real-time RT-PCR assay. NTS was absent in the normal liver cell L02, but was expressed exclusively in the HCC cells. In these cells, Hep3B and HepG2 produced more NTS than the others did. b. The expression level of NTR1 was detected using Western blot. HepG2 cells expressed a higher level of NTR1 protein than the Hep3B cells did, indicating that stronger NTS signal in HepG2 cells than in Hep3B cells. c. The migration capacity of 4 HCC cell lines was analyzed using wound healing test, and HepG2 cells displayed a higher migration capacity than Hep3B cells did. d. The invasion potential of 4 HCC cell lines was evaluated through Transwell invasion assay, and HepG2 cells displayed higher invasion potentials than Hep3B cells did.