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. 2016 Sep 7;7(43):70404–70419. doi: 10.18632/oncotarget.11879

Figure 1. Ectopic expression of AR conferred functional AR-mediated androgen signaling in NHPrE1 cells.

Figure 1

Retroviral vector pLNCX or pLNCX-AR was used to generate NHPrE1 cells with empty vector (EV) control or AR transgene. A. Western blot to analyze the expression of AR in NHPrE1/EV (EV) or NHPrE1/AR (AR) cells. Beta-actin served as a loading control. B-D. quantitative (q)RT-PCR to assess the levels of AR (B) and androgen responsive genes PSA (C) and FKBP5 (D). Androgen treatment (DHT, 10 nM) induced the expression of PSA and FKBP5. The expression of GAPDH was used to normalize the qPCRs. E. immunofluorescence staining of AR. NHPrE1/AR cells were cultured in androgen-depleted medium for 24 hours and then treated with R1881 (1nM) for 2 or 4 hours. Immunofluorescence staining of AR was conducted to examine the nuclear translocation of AR upon androgen treatment.