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. 2016 Sep 7;7(43):70404–70419. doi: 10.18632/oncotarget.11879

Figure 2. Androgen differentially regulated prostatic cell proliferation.

Figure 2

The proliferation of NHPrE1 cells with or without AR expression was assessed by using both WST-1 A. and IncuCyte B. methods. NHPrE1/EV cells and NHPrE1/AR cells were cultured in the absence or presence of androgens (10 nM DHT or 1 nM R1881). Androgen treatment had negligible effects on the proliferation of empty vector control cells, but suppressed proliferation of AR-expressing NHPrE1 cells (panel B, p<0.01 from 36 hour onward, comparison between ethanol- and R1881- treated NHPrE1/AR cells). Overall, compared with NHPrE1/EV cells, NHPrE1/AR cells displayed suppressed cell proliferation. C and D, blocking AR attenuated androgen-induced proliferation inhibition. NHPrE1/AR (C) or PC3/AR cells (D) were cultured with or without androgen (10 nM DHT) in the presence or absence of 10 μM bicalutamide (Bic) for 5 days. While DHT suppressed the proliferation of NHPrE1/AR and PC3/AR cells, addition of bicalutamide attenuated this inhibitory effect of androgens. *p<0.05, t-test.