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. 2016 Sep 13;7(43):70462–70474. doi: 10.18632/oncotarget.11992

Figure 1. Gene expression changes in TRAMP prostate through disease progression.

Figure 1

qPCR levels of Runx1 (A), Runx2 (B), Histone H4 (C), androgen receptor (AR) (D), αvβ6-Integrin (E), Nkx3.1 (F), Pten (G), Tgf-β1 (H), and p21 (I) message in the ventral and lateral prostate lobes isolated from wild type (•) or TRAMP (□) animals. Animals were sacrificed at 6, 12, 16, 21, 28, and 33 weeks to follow tumor development. Both Runx1 (A) and Runx2 (B) transcriptional drivers of tumor progression are elevated in TRAMP prostates irrespective of animal age and increase throughout the time course. (A-C) Relative quantity calculated as 1/(2^(Ct,gene – Ct,18S)). (D-I) Relative expression calculated using the delta delta Ct method, 2^((Ct,gene – Ct,18S)wild type – (Ct,gene – Ct,18S)TRAMP), at each time point. All expression values normalized to 18S. N = 3 animals per time per genotype. Error bars are SEM. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to wild type.