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. 2016 Sep 16;7(43):70546–70558. doi: 10.18632/oncotarget.12060

Figure 2. Deptor modulates transcription of genes involved in ER homeostasis.

Figure 2

A. WB analysis with the indicated Abs of total cell extracts (TCEs) from KMS27 cells transiently transfected with Stealth siRNA negative control (siControl) or siRNA Deptor (siDeptor). B. Volcano Plot of all assembled RNA-seq genes performed with KMS27 cells transiently transfected as in A. The image depicts genes with strong modulation but not significant (qvalue < 0.05) in orange; significant modulation but mild fold-change (abs (log2fc) < 1) in red; significant and strongly modulated genes in green. C. Gene Ontology significance barplot of all siDeptor modulated genes deriving from Cuffdiff analysis. D. Intensity (FPKM) heatmap of endoplasmic reticulum genes (top) and ER stress genes (bottom) modulated after Deptor silencing. E. Correlation analysis of expression values from 550 myeloma patients, as described in Zhan et al [28], between Deptor and endoplasmic reticulum genes (ERLIN2, KEAP1, PSEN2 and DERL3) (probe set 218858_at for DEPTOR). R2 values: Erlin2=0,234; KEAP1=−0,118; PSEN2=−0,318; DERL3=−0,136.