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. 2016 Sep 16;7(43):70546–70558. doi: 10.18632/oncotarget.12060

Figure 3. Deptor modulates transcription of genes involved in ER homeostasis.

Figure 3

A-B. Quantitative RT–PCR (qRT–PCR) for ER homeostasis gene expression was performed in KMS27 (A) and KMS18 (B) cells transiently transfected with Stealth siRNA negative control (siControl) or siRNA Deptor (siDeptor). Values were normalized to RPL19 mRNA expression. Error bars represent the standard error of three different experiments. *P = 0.002, **P≤0.03 (A); *P≤0.0002, **P≤0.01 (B). C. WB analysis with the indicated Abs of TCEs from KMS27 and KMS18 cells transfected as in A and B. Arrowhead indicates specific ERLIN2 protein band. D. ChIP-qPCR analysis of KMS27 cells using anti-Deptor Ab or control IgGs. Primer were designed to amplified two different promoter regions of CKAP4, ERLIN2 and PSEN2. Error bars represent the standard error of three different experiments. n.s., not significant, *P≤0.0004, **P=0.0225.