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. 2016 Sep 16;7(43):70546–70558. doi: 10.18632/oncotarget.12060

Figure 5. Deptor sensitizes MM cells to Bz treatment.

Figure 5

A. Top: WB analysis with the indicated Abs of TCEs from KMS27 cells treated or not with different concentrations of Bz for 4hrs and 8hrs. Bottom: qRT-PCR for Deptor mRNA expression of KMS27 cells treated as in A. Values were normalized to RPL19 mRNA expression. Error bars represent the standard error of three different experiments performed in duplicate. n.s., not significant, *P<0.03. B. WB analysis with the indicated Abs of TCEs from KMS27 cells transfected with Stealth siRNA negative control (siControl) or siRNA Deptor (siDeptor) and after 24hrs treated or not with 5nM Bz for further 24 and 48hrs. C. Cell death detection of KMS27 cells treated like in B and assayed by trypan blue staining. Percentages represent trypan blue-incorporating cells. Data are presented as the mean SD from three independent experiments performed in duplicate. *P=0.0433, **P≤0.01. D. MM cells overexpressing (KMS27 and KMS18) or lacking (ARH77) of Deptor expression were treated with the indicated concentrations of Bz for 48hrs and cell death was assayed by trypan blue staining. Percentages represent trypan blue-incorporating cells. Data are presented as the mean SD from three independent experiments performed in duplicate. n.s., not significant, *P≤0.01, **P≤0.03.