Figure 1. Evaluation of cytotoxic effect in response to Ibrutinib and Nutlin-3 used alone or in combination in B leukemic cell lines.

The p53^wild-type (EHEB, JVM-2, JVM-3) and the p53^mutated (MEC-1, MEC-2) leukemic cells were exposed to Ibrutinib (10 μM) and Nutlin-3 (10 μM) used either alone or in combination for 48 hours. Cell viability was calculated as percentage with respect to the control untreated cultures (set to 100% for each cell line). Data are reported as the mean±SD of results from at least three independent experiments. The asterisk indicates p<0.05 respect to single treatments.