Figure 4. Characterization of MCTs expression and other glycolytic markers in CRC cells after acetate treatment.

HCT-15 and RKO cells were incubated with acetate (45 mM and 70 mM for HCT-15 cells; 75 mM and 110 mM for RKO cells, respective doses for the IC₃₀ and IC₅₀) or with fresh medium (as negative control) for 24 and 48 hours. Western blotting images of the SMCT1, MCT1, MCT4, MCT2, CD147 and GLUT-1 expression. β-actin was used as loading control. A representative experiment of at least three independent experiments is shown.