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. 2016 Sep 27;7(43):70881–70897. doi: 10.18632/oncotarget.12282

Figure 7. PAK4 mediates invadopodia dynamics via PDZ-RhoGEF.

Figure 7

A. Immunoprecipitation in HEK293 cells of GFP-PAK1/PAK4 in the presence/absence of myc-PDZ-RhoGEF expression with a myc-PDZ-RhoGEF alone control. The Immunoprecipitation was probed for presence of myc-PDZ-RhoGEF B. Representative images of PAK4 knockdown WM-115 cells expressing myc-PDZ-RhoGEFΔDH. Scale bars = 10μm. The percentage of cells with invadopodia C. the area of degradation D. and the percentage of cells with prominent actin fibres E. in PAK4 knockdown cells expressing myc-PDZ-RhoGEFΔDH. Significance was calculated to control WM-115 cells. Data are mean values ± S.E.M. of 150 (Percentage of invadopodia and prominent actin fibres) or 90 (Degradation) cells, over 3 independent experiments; * = P 00< 0.05. F. Representative images of WM-115 cells expressing myc-PDZ-RhoGEF. Scale bars = 10μm. The percentage of cells with invadopodia G. the area of degradation H. and the percentage of cells with prominent actin fibres I. in cells expressing myc-PDZ-RhoGEF. Significance was calculated to wildtype WM-115 cells. J. Co-localisation of GFPPAK4 and myc-PDZ-RhoGEF (wildtype and ΔDH mutant) with F-actin and TRITC gelatin degradation. Scale bar = 10μm.