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. 2016 Sep 27;7(43):70898–70911. doi: 10.18632/oncotarget.12286

Figure 3. GPR81 activation promotes angiogenesis in primary human endothelial cells in vitro.

Figure 3

A. Angiogenic factor array of the conditioned media (CM) from MCF7-shCTL (top) and MCF7-shGPR81 cells (bottom) (left). The relative signal intensity of the indicated factor is presented (right). The data are expressed as the mean±SEM; n≥3. B. AREG levels in the CM from MCF7-shCTL and MCF7-shGPR81 cells were measured via an enzyme-linked immunosorbent assay (ELISA). *, P<0.005. The data are expressed as the mean±SEM; n≥3. C. AREG mRNA expression was measured via quantitative real-time PCR. All data are shown as the mean±SEM; n≥3; *, P<0.005. D. Representative images of HUVEC tube formation. HUVECs were treated with recombinant AREG at different concentrations. The bars represent the mean value of the tube area±SEM from five independent experiments (right). Scale bar, 20 μm; **, P<0.01. E. Representative images of HUVEC tube formation in the indicated conditions. Tube formation was quantified in five randomly selected fields (right). Scale bar, 20 μm; *, P<0.01; **, P<0.05.