Figure 2A–2D.

Effect of XIST knockdown on the growth and apoptosis of lung cancer cells. (A) We designed siRNA for different gene loci of XIST gene and detected the knockdown effect by real-time PCR. (B) Growth curves of A549 and H1299 cells after transfection with siXIST or siNC were determined by CCK8 assays. (C) The effect of XIST knockdown on apoptosis in A549 and H1299 cells was determined by measuring the percentage of Annexin V-stained cells using flow cytometry. (D) Representative images (left) and quantification (right) of the colony formation assay of A549 and H1299 cells transfected with siXIST or siNC. Mean±SD. ^*P<0.05, ^**P<0.01.