Figure 3.

Effect of knockdown of XIST gene on cell metastasis. (A) Wound healing assay was used to demonstrate the effects of XIST knockdown on migration of A549 and H1299 in vitro. (B, C) Transwell assay was used to detect cell migration and invasion with knockdown of XIST. (D) Fluorescence detection format were used in transwell assay with matrigel to evaluate the cell invasion ability. (E) Effect of XIST knockdown on expression of E-cadherin detected by Western blot in A549 and H1299 cells. Each treatment was performed with at least three biological replicates. Mean±SD. ^*P<0.05, ^**P<0.01.