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. 2017 Jan 23;38(3):342–350. doi: 10.1038/aps.2016.141

Figure 6.

Figure 6

Dose-dependent reduction of iNOS, COX-2, and Myd88 expression by LYRM03 in LPS-activated alveolar macrophages. Alveolar macrophages were collected and incubated with or without LYRM03 (0, 50, 100, and 500 μmol/L) for 1 h. Cells were treated with LPS (100 ng/mL) for 16 h. Control cells were incubated with the cell culture medium. (A) Western blot analysis of the iNOS, COX-2, and Myd88 proteins in alveolar macrophages. β-Actin was used as the loading control. (B) The ratios of iNOS, COX-2, and Myd88 to β-actin are shown in the densitometry. (C) The culture supernatant was collected, and the NO levels were measured using Griess reagent at 24 h. Statistical analyses were performed with Student's t-test. *P<0.05, **P<0.01. ns (not significant). n= 3.