Figure 7.

LYRM03 suppressed IκB degradation and p38 activation in alveolar macrophages. Alveolar macrophages were pre-incubated with or without LYRM03 (100 μmol/L) for 1 h. Cells were subsequently stimulated with LPS (100 ng/mL) for 0, 5, 15, 30, 45, and 60 min. Control cells were incubated with the cell culture medium. (A) Western blotting for IκB, phosphor-p38 (p-p38) and total p38 (t-p38) in alveolar macrophages. β-actin was used as the loading control. (B) The ratios of IκB to β-actin and phosphor-p38 (p-p38) to total-p38 are shown in the densitometry. Statistical analyses were performed with Student's t-test. ^*P<0.05, ^**P<0.01. n=4.