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. 2016 Oct 11;7(46):74473–74483. doi: 10.18632/oncotarget.12578

Figure 7. Effect of 7-KC on cellular ROS levels and Akt activation.

Figure 7

A. 7-KC (10-40 μg/ml) induced ROS production as shown by increase in cellular DCF fluorescence. Results were expressed as Mean ± SE (% of control, n = 3), B. 7-KC stimulate Akt phosphorylation as shown by increase in cellular p-Akt fluorescence (blue - DAPI, red - p-Akt). One representative immunofluorescent study was shown. C. LY294002 attenuated the 7-KC-induced apoptosis of endothelial cells as analyzed by PI+Annexin V dual staining flow cytometry. Results were expressed as percentage (% of control, n = 6) of cell residing in UL, LL, UR and LR area of flow cytometry chart. D. LY294002 prevented the 7-KC-induced IL-8 mRNA expression of endothelial cells.