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. 2016 Oct 21;7(46):74496–74509. doi: 10.18632/oncotarget.12812

Figure 2. Immunofluorescent analysis of the fibroblast cultures from the KD patients.

Figure 2

A. Control and both KD1 and KD2 fibroblasts were fixed, permeabilized, and stained. LAMP1 (green) and LAMP2 (red) are both expressed higher in the patients compared to a representative healthy control fibroblasts. Enlarged images of the regions highlighted by dashed white squares in merged images (left) are in the right columns. DNA is identified by DAPI. Scale bars: 25 μm. B. Representative STED images of LAMP1 morphology differences in healthy controls and KD fibroblasts. LAMP1 morphologies are altered with enlarged and fragmented LAMP1-positive vesicles. Nuclei are indicated by asterisks (*). Scale bars: 5 μm. C.-E. The increased distribution ranges of lysosome diameters in patient fibroblasts (D, KD1: range, 0.74-4.15 μm; E, KD2: range, 0.75-4.68 μm) compared to healthy control fibroblasts (C, CTL: range, 0.36-1.03 μm). For each plot, n = 20 cells.