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. 2016 Jul 24;7(46):74658–74677. doi: 10.18632/oncotarget.10813

Figure 6. CSNPs-altered epigenetics in preimplantation embryos.

Figure 6

A. Real time qRT-PCR analysis of the Tet family (Tet-1, Tet-2, Tet-3) and B. the Dnmt family (Dnmt1, Dnmt3a, Dnmt3b). C. 5-mC to 5-hmC analysis. D. Immunostaining of 5-mC and 5-hmC. The fluorescence intensities of 5-mC and 5-hmC were measured in both the ICM and the TE. E. Heterochromatin condensation was observed in the control and CNSPs-treated groups using TEM. To calculate each type of heterochromatin and based on the condensation, we divided the embryos into 3 groups: Types I, II and III. Transcriptional silencing is associated with the targeting of genomic sequences to repressive (heterochromatic) nuclear compartments. F. The H3K27me3 expression level was analyzed using immunofluorescence staining. Embryos were divided into Types I, II, and III by their expression patterns. G. The relative expression levels of methylase (Ezh2) and its co-factors (Eed5, Suz12) were measured using real time qRT-PCR analysis. *, **, and NS indicate p<0.05, p<0.01, and not significant, respectively. RM indicates rapamycin.