Figure 1. More maturation and Enhanced effector DC differentiation in iNOS-deficient mice.

A. Bone marrow cells from wild type or iNOS^−/− mice were cultured with GM-CSF (10ng/ml) and IL-4 (10ng/ml) for 7 days, then stimulated with IFN-γ (10ng/ml) plus LPS (100ng/ml) for 24 h, maturation markers including MHC II, CD80 and CD86 expression in CD11b⁺CD11c⁺ cells were analyzed by FACS. B. Cells prepared in (A) were intracellular and surface stained for molecules of effector and regulatory DC in CD11b⁺CD11c⁺ cells by FACS. C. The cells prepared in (A) and iNOS expression in CD11b⁺CD11c⁺ cells was determined by FACS. D. The purity of CD11b⁺CD11c⁺ cells population in (A) were analyzed by FACS for cell surface staining. E. The cells prepared in (A) and mRNA expression of indicated genes was determined by qPCR. F. The supernatants in (A) were analyzed by ELISA. Data represent mean ± SD. * P<0.05. **P<0.01.