Figure 4. iNOS deficiency promotes the effector DC differentiation in vivo.

A. WT or iNOS^−/− mice were injected (i.p) with LPS for 24 hours. Spleen morphology and mice survival rate was observed. B. Total RNA from spleen of WT or iNOS^−/− mice after LPS injection as in (A) was extracted for qPCR. C. Effector/stimulatory DC marker in CD11b⁺CD11c⁺ prepared from spleens of WT or iNOS^−/− mice after LPS injection as in (A) and analyzed by FACS. D. Maturation markers in CD11b⁺CD11c⁺ cells of mesenteric lymph node of WT or iNOS^−/− mice after LPS injection as in (A) were analyzed by FACS. Data represent mean ± SD. * P<0.05. **P<0.01.