Table 2. Oligonucleotides used in this study.
Gene | Forward primer | Reverse primer | Tan | Cycles |
---|---|---|---|---|
APAF1 | ACAATGCTCTACTACATGAA GGATATAAAGA | CACTGGAAGAAGAGACAACAGGAA | 60°C | 40 |
ARID1A | TCTTGCCCATCTGATCCATT | CCAACAAAGGAGCCACCAC | 60°C | 40 |
ATF3 | AAGAACGAGAAGCAGCATTTGAT | TTCTGAGCCCGGACAATACAC | 60°C | 40 |
CDKN1A | CCTCATCCCGTGTTCTCCTTT | GTACCACCCAGCGGACAAGT | 60°C | 40 |
DHRS2 | CTCCATGTAGGGCAGCAACT | GTAGGGAGCACTCTGGGGAC | 60°C | 40 |
DUSP1 | GTACATCAAGTCCATCTGAC | GGTTCTTCTAGGAGTAGACA | 60°C | 40 |
FAS / APO-1 | AGCTTGGTCTAGAGTGAAAA | GAGGCAGAATCATGAGATAT | 60°C | 40 |
FOS | GAGAGCTGGTAGTTAGTAGCATGTTGA | AATTCCAATAATGAACCCAATA GATTAGTTA | 60°C | 40 |
GADD45B | GTCGGCCAAGTTGATGAAT | CACGATGTTGATGTCGTTGT | 60°C | 40 |
GADD45B genotyp. | CCCGTCACTGATCCCTCTTT | CTACTGCGAAGAAAGCCGG | 55°C | 35 |
GAPDH | TGCCAAATATGATGACATCAAGAA | GGAGTGGGTGTCGCTGTTG | 60°C | 40 |
HDAC1 | TAAATTCTTGCGCTCCATCC | AACAGGCCATCGAATACTGG | 60°C | 40 |
HDAC2 | CGTGTAATGACGGTATCATTCC | ACCAGATAATGAGTCTGCACC | 60°C | 40 |
HDAC3 | CTGTGTAACGCGAGCAGAAC | GCAAGGCTTCACCAAGAGTC | 60°C | 40 |
ID2 | TCAGCCTGCATCACCAGAGA | CTGCAAGGACAGGATGCTGATA | 60°C | 40 |
P53 | TTGCAATAGGTGTGCGTCAGA | AGTGCAGGCCAACTTGTTCAG | 60°C | 40 |
RHOB | GGGACAGAAGTGCTTCACCT | CGACGTCATTCTCATGTGCT | 60°C | 40 |
SMARCA4 | CAGCATGCCAAGGATTTCAAG | CGATCCGCTCGTTCTCTTTC | 60°C | 40 |
SMARCB1 | AACGTCAGCGGGTTCAAAT | GCCTTCACCTGGAACATGAA | 60°C | 40 |
ZMYND11 | TTGTTAAACGTGCCATGACC | GCATGTGTGGAGACAGAGGA | 60°C | 40 |
Gene | shRNA sequence | |||
---|---|---|---|---|
ARID1A sh1 | 5'-TCGCCTCTATGTGTCTGTGAAGGAGATTG-3' | |||
ARID1A sh2 | 5'-GCAAACTCAGCATCCAGGACAACAATGTG-3' | |||
ARID1A sh3 | 5'-CCTTGCTGTCTCAGCAGCCAATCAACTTA-3' | |||
ARID1A sh4 | 5'-CCATTTGTGGTGGACTGCTCAGATAACGT-3' |
Gene | gRNA sequence | |||
---|---|---|---|---|
GADD45B gRNA 1 | 5'-CACAGTGGGGGTGTACGAGT-3' | |||
GADD45B gRNA 2 | 5'-ACAACGACATCAACATCGTG-3' | |||
GADD45B gRNA 3 | 5'-CTCTTCCAGGCGTCCGTGTG-3' |