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. 2016 Aug 27;7(46):74931–74946. doi: 10.18632/oncotarget.11647

Table 2. Oligonucleotides used in this study.

Gene Forward primer Reverse primer Tan Cycles
APAF1 ACAATGCTCTACTACATGAA GGATATAAAGA CACTGGAAGAAGAGACAACAGGAA 60°C 40
ARID1A TCTTGCCCATCTGATCCATT CCAACAAAGGAGCCACCAC 60°C 40
ATF3 AAGAACGAGAAGCAGCATTTGAT TTCTGAGCCCGGACAATACAC 60°C 40
CDKN1A CCTCATCCCGTGTTCTCCTTT GTACCACCCAGCGGACAAGT 60°C 40
DHRS2 CTCCATGTAGGGCAGCAACT GTAGGGAGCACTCTGGGGAC 60°C 40
DUSP1 GTACATCAAGTCCATCTGAC GGTTCTTCTAGGAGTAGACA 60°C 40
FAS / APO-1 AGCTTGGTCTAGAGTGAAAA GAGGCAGAATCATGAGATAT 60°C 40
FOS GAGAGCTGGTAGTTAGTAGCATGTTGA AATTCCAATAATGAACCCAATA GATTAGTTA 60°C 40
GADD45B GTCGGCCAAGTTGATGAAT CACGATGTTGATGTCGTTGT 60°C 40
GADD45B genotyp. CCCGTCACTGATCCCTCTTT CTACTGCGAAGAAAGCCGG 55°C 35
GAPDH TGCCAAATATGATGACATCAAGAA GGAGTGGGTGTCGCTGTTG 60°C 40
HDAC1 TAAATTCTTGCGCTCCATCC AACAGGCCATCGAATACTGG 60°C 40
HDAC2 CGTGTAATGACGGTATCATTCC ACCAGATAATGAGTCTGCACC 60°C 40
HDAC3 CTGTGTAACGCGAGCAGAAC GCAAGGCTTCACCAAGAGTC 60°C 40
ID2 TCAGCCTGCATCACCAGAGA CTGCAAGGACAGGATGCTGATA 60°C 40
P53 TTGCAATAGGTGTGCGTCAGA AGTGCAGGCCAACTTGTTCAG 60°C 40
RHOB GGGACAGAAGTGCTTCACCT CGACGTCATTCTCATGTGCT 60°C 40
SMARCA4 CAGCATGCCAAGGATTTCAAG CGATCCGCTCGTTCTCTTTC 60°C 40
SMARCB1 AACGTCAGCGGGTTCAAAT GCCTTCACCTGGAACATGAA 60°C 40
ZMYND11 TTGTTAAACGTGCCATGACC GCATGTGTGGAGACAGAGGA 60°C 40
Gene shRNA sequence
ARID1A sh1 5'-TCGCCTCTATGTGTCTGTGAAGGAGATTG-3'
ARID1A sh2 5'-GCAAACTCAGCATCCAGGACAACAATGTG-3'
ARID1A sh3 5'-CCTTGCTGTCTCAGCAGCCAATCAACTTA-3'
ARID1A sh4 5'-CCATTTGTGGTGGACTGCTCAGATAACGT-3'
Gene gRNA sequence
GADD45B gRNA 1 5'-CACAGTGGGGGTGTACGAGT-3'
GADD45B gRNA 2 5'-ACAACGACATCAACATCGTG-3'
GADD45B gRNA 3 5'-CTCTTCCAGGCGTCCGTGTG-3'