Figure 3. Validation of selected proteins in human breast cancer non-metastatic cell line MCF7 and highly metastatic cell line MDA-MB-231.

A. Nanoparticle Tracking Analysis (NTA) of particles secreted from MCF7 and MDA-MB-231 cell lines. The relative concentration of secreted particles (10⁶ particles/ml), which obtained from Nanosight was normalized with mg of proteins of cell lysates (means +/− SD, n=3, 5×1 minutes movies sequences were measured). B. Western blot analysis of MCF7 and MDA-MB-231 cells and exosomes, probed for the indicated proteins (n=2).