Figure 4. Overexpression of miR-885-5p inhibits proliferation of HCCLM3 cells in vivo and prolongs the survival of tumor-bearing Mice.

(A–D) HCCLM3-lv- miR-885-5p or HCCLM3-lv-NC tumor-bearing mice were euthanized after 43 days, and the lungs were histologically examined for metastatic foci. (A), miR-885-5p expression levels of HCCLM3-lv-miR-885-5p and HCCLM3-lv-NC cells. (B), Ex vivo imaging analysis of transfected HCCLM3 cells indicates increasing bioluminescence signals with increasing cell number (R² = 0.9904). (C), In vivo imaging of tumors over 43 days. (D), Number (mean ± SD) of lung metastatic foci was determined from five serial sections. Values shown are expressed as mean ± SD. The scale bars = 100 μm. *P < 0.05. (E), Survival curve for mice injected with HCCLM3 cells expressing miR-885-5p or NC control (negative control) as determined by Kaplan-Meier analysis.