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. 2016 Sep 13;7(46):75064–75080. doi: 10.18632/oncotarget.11991

Figure 6. Co-immunoprecipitation experiment reveals that PEDV nucleoprotein interacts with C/EBP-β.

Figure 6

A. Vero cells were infected with PEDV (MOI=0.1) for 24h. The cells were lysed with the lysis buffer containing DNase (100μg/ml) and RNase A(100μg/ml). Immunoprecipitation (IP) experiment was performed with anti-N antibody or control rabbit immunoglobulin G (IgG), followed by immunoblot analysis (IB) with C/EBP-β or NF-κB antibody. B. Vero cells were first transfected with PCA-N for 24h. Immunoprecipitation (IP) experiment was performed with anti-HA antibody or control rabbit immunoglobulin G (IgG), followed by immunoblot analysis (IB) with C/EBP-β or NF-κB antibody. C. The colocalization between C/EBP-β, NF-κB and PEDV-N was studied under confocal microscopy.