Table 1. Sequences of the primers used for cloning and qRT-PCR.
Name | Sequence |
---|---|
PEDV-N | Forward-GCACCGCGGCCGCATG GCT TCT GTC AGC TTT CAG G Reverse -GCACCTCTAGAATT TCC TGT GTC GAA GAT CTC G |
pHLuc-HMGB1 | Forward-GCACCGGTACCATAATCCCTGAGTCCGATAGTGTA Reverse-GCACCAGATCTTTATGCTCCTCCCGACAAG |
pHLuc-HMGB1-1 | Forward-GCACCGGTACCCCACTACAGCCAGCACATTT Reverse-GCACCAGATCTTTATGCTCCTCCCGACAAG |
pHLuc-HMGB1-2 | Forward-GCACCGGTACCGACATGCTGTGAAACTCGA Reverse-GCACCAGATCTTTATGCTCCTCCCGACAAG |
pHLuc-HMGB1-3 | Forward-GCACCGGTACCTTCGCTCCGGCGGCCGCTC Reverse-GCACCAGATCTTTATGCTCCTCCCGACAAG |
PEDV-ORF3 | Forward-TTTGCACTGTTTAAAGCGTCT Reverse-AGTAAAAGCAGACTAAACAAAGCCT |
PEDV-N | Forward-GCACTTATTGGCAGGCTTTGT Reverse-CCATTGAGAAAAGAAAGTGTCGTAG |
GAPDH | Forward-AGGTCGGAGTCAACGGATTT Reverse-TAGTTGAGGTCAATGAAGGG |
IL-1β | Forward GGAAGACAAATTGCATGG Reverse CCCAACTGGTACATCAGCAC |
IL-6 | Forward AGAGGCACTGGCAGAAAAC Reverse TGCAGGAACTGGATCAGGAC |
IL-8 | Forward AGGACAAGAGCCAGGAAGAA Reverse ACTGCACCTTCACACAGAGC |
TNF-α | Forward TCTGTCTGCTGCACTTTGGAGTGA Reverse TTGAGGGTTTGCTACAACATGGGC |