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. 2016 Sep 17;7(46):75155–75164. doi: 10.18632/oncotarget.12090

Figure 1. ASK1 is overexpressed in pancreatic cancer.

Figure 1

A, B. Representative images showing immunohistochemical staining of ASK1 expression in pancreatic cancer tissues (A) and adjacent tissues (B). C. Quantification of ASK1 expression in pancreatic cancer tissues (n = 19) and adjacent tissues (n = 10). D, E. Western blot analysis (D) and quantification (E) of ASK1 expression in normal pancreatic epithelial cells and pancreatic cancer cell lines. Experiments were repeated 3 times. F, G. RT-PCR analysis (F) and quantification (G) of ASK1 mRNA expression in normal pancreatic epithelial cells and pancreatic cancer cell lines. NC, negative control (i.e., without RNA). Experiments were repeated 3 times. Error bars represent mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.