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. 2016 Sep 17;7(46):75155–75164. doi: 10.18632/oncotarget.12090

Figure 3. ASK1 knockdown impairs pancreatic cancer cell proliferation.

Figure 3

A. Western blot analysis of ASK1 and α-tubulin expression in PANC1 cells transfected with control or ASK1-targeted siRNAs. B-D. PANC1 cells were transfected with control or ASK1-targeted siRNAs, and cell proliferation was examined by SRB (B), MTT (C), and CCK8 (D) assays. E. Western blot analysis of ASK1 and α-tubulin expression in AsPC1 cells transfected with control or ASK1-targeted siRNAs. F, G. AsPC1 cells were transfected with control or ASK1-targeted siRNAs, and cell proliferation was examined by SRB (F) and MTT (G) assays. H. PANC1 cells were transfected with control or ASK1-targeted siRNAs, and the percentage of apoptotic cells was examined by staining with annexin V-FITC and propidium iodide followed by flow cytometry. I, J. Representative images from colony formation assays (I) and quantification of colonies (J) derived from PANC1 cells transfected with the indicated siRNAs. Error bars represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant.