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. 2016 Sep 17;7(46):75155–75164. doi: 10.18632/oncotarget.12090

Figure 5. Effect of ASK1 on the motility of pancreatic cancer cells.

Figure 5

A. PANC1 cells were transfected with the indicated siRNAs, and cell migration was examined by wound healing assays. B. Quantification of (A). The relative migration area at 16 hours after scratch creation was measured. C. Transwell assays were performed using PANC1 cells transfected with control or ASK1 siRNAs in the absence or presence of matrigel on the upper surface of the transwell filters. D, E. Migratory (D) and invasive (E) abilities of pancreatic cancer cells were quantified based on experiments shown in the upper and lower panels, respectively in (C). F, G. PANC1 cells were transfected with plasmids expressing HA, HA-ASK1, or HA-ASK1-KD, and cell migration was analyzed using wound healing assays. Error bars represent mean ± SEM. ns, not significant.