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A. Mouse hepatocytes were fixed and stained for c-Myc and nucleostemin (GNL3L) in combination with DAPI; B. Immunofluorescence image cytometry analysis showed that c-Myc and GNL3L protein content per genome is significantly higher in tetraploid cell nuclei as compared with diploid and C. RT-PCR performed with two different primer pairs, confirms expression of c-Myc and GLN3: 35, 40: PCR cycles number.
