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. 2016 Sep 21;7(46):75328–75338. doi: 10.18632/oncotarget.12175

Figure 2. Cell viability and activation of TP53 target proteins in clear cell carcinomas treated with RG7112.

Figure 2

A and B. Cell viability, as measured by MTT assay, in cells with wild type (A) or mutant (B) TP53. Experiments were repeated three times. C. Differences in cell viability in cell lines with wild type or mutant TP53 and exposed to indicated concentrations of RG7112. Group means were compared by paired t-test. D and E. Time- (D) and dose-dependent (E) accumulation of MDM2, TP53, and TP21, as measured by western blotting. RG7112 was added at 2.5 μM in (D). F. Induction of TP53 phosphorylation and TP53 target proteins (TP21 and PUMA), as determined by western blotting. Cleaved PARP and survivin were also assessed to detect proapoptotic signaling.