Skip to main content
. 2016 Sep 26;7(46):75539–75550. doi: 10.18632/oncotarget.12265

Figure 3. SC09 suppresses JAK2 phosphorylation.

Figure 3

A. NCI-H929 and RPMI-8226 cells were treated with SC09 at indicated concentrations overnight, followed immunoblotting assay against kinases as indicated. B. SC09 was docked into the ATP-binding pocket of JAK2 by computer modeling. C. SC09 formed extensive van der Waals interactions with the hydrophobic residues of JAK2. D. SC09 formed strong and stable hydrogen bonds with Leu855 of JAK2 (in Red). E. NCI-H929 and RPMI-8226 cells were starved overnight before SC09 treatment at indicated concentrations for 4 h and IL-6 (50 ng/ml) stimulation for 20 min, followed by analysis of JAK2 activation. F. RPMI-8226 cells were treated with SC09 at 20 μM for different time points, followed by immunoblotting assay against phosphorylated JAK2.