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. 2016 Sep 26;7(46):75539–75550. doi: 10.18632/oncotarget.12265

Figure 4. SC09 induces MM cell apoptosis by suppressing STAT3 activation.

Figure 4

A. The expression profile of p-STAT3 in MM cell lines and NIH-3T3 cells. B. MM and NIH-3T3 cells were treated with SC09 at indicated concentrations for 24 h, and cell viability was measured by trypan blue staining. C. MM cell lines were treated with SC09 for 24 h followed by an immunoblotting assay to evaluate the cleavage of PARP. D. RPMI-8226 cells were treated with SC09 at indicated concentrations with or without 50 ng/ml of IL-6 for 24 h. Cells were harvested and prepared for immunoblotting against specific antibodies. E. Myc-STAT3 plasmids or empty vectors were transfected into RPMI-8226 for 24 h, followed by SC09 treatment at 10 or 20 μM for 24 h. Cells were harvested and prepared for immunoblotting against PARP, p-STAT3 and Myc.